Fish Encapsulated yeast strain could boost immune response in fish
Sản phẩm khuyên dùng
Máy thổi khí AT thiết kế nhỏ gọn, lưu lượng khí lớn, áp suất mạnh. Phù hợp để lắp đặt sục khí ao nuôi tôm, trại giống, ương tôm, nuôi tôm nhà màng …
Sản phẩm khuyên dùng
Tima - Tủ điều khiển ứng dụng mạng không dây LoRa. Tima sử dụng giao thức truyền dữ liệu không dây tầm xa LoRa điều khiển thiết bị như máy bơm nước, động cơ điện…
Sản phẩm khuyên dùng
Chúng tôi tự hào giới thiệu Ống thông khí Nano-Tube bọt khí mịn, oxy hoà tan cao, cải tiến quan trọng để khuếch tán oxy hoà tan trong nước phục vụ nuôi tôm công nghệ cao.

Encapsulated yeast strain could boost immune response in fish

Author Aerin Curtis, publish date Friday. December 29th, 2017

Encapsulated yeast strain could boost immune response in fish

Freeze-dried, encapsulated yeast may boost tilapia growth, health and intestinal structure, say researchers from Thailand.

Their study published in Research in Veterinary Science​ suggests a protected form of Saccharomyces​ cerevisiae JCM 7255​ could be a potential probiotic strain for use in tilapia culture and that the encapsulation technique used improved the viability of yeast during processing and storage.

“Taken together, the data from these experiments show that a combination of alginate encapsulation and 10% skim milk is suitable to improve the viability of S. cerevisiae during storage and during exposure to simulated gastro-intestinal condition,”​ the scientists said. “Encapsulated S. cerevisiae JCM 7255 can improve intestinal structure and growth performance in tilapia. Induction of gut immune parameters and inhibition against streptococcal bacteria were also noted.”​

Tilapia production is rapidly growing as the species is recognized as an important freshwater fish protein source for human consumption, said the authors. “In order to meet consumer demand, intensive tilapia culture is expanding all over the world. At the same time, outbreaks of infectious diseases, including streptococcosis, have been described in tilapia.”​

Yeast candidature ​

The researchers note that, among probiotic candidates, bacteria are recognized as the most common probiotic microorganisms.

“However the transfer of antibiotic resistance genes among probiotic bacteria is widely mentioned by scientific researchers. Other microorganisms such as yeasts are increasingly being considered as alternative promising candidates for probiotics,”​ they added.

And several S. cerevisiae​ strains, said the Thai team, contain enzymes, fatty acids, vitamins and amino acids.

“Their immunostimulant components such as beta glucans, nucleic acids and mannan oligosaccharides have been shown to activate the immune response,”​ said the researchers.

Delivering yeast supplements​

One challenge to using live yeast as a probiotic is ensuring that the organism survives until it reaches the intestinal tract, said researchers. The probiotic was tested in encapsulations of an alginate matrix and the matrix with 10% skim milk and tested against simulated gastric liquid and bile. 

“The viability of encapsulated S. cerevisiae in skim milk-alginate capsules was significantly higher than in alginate capsules,”​ the researchers said. But, both types of encapsulation survived longer at room temperature and in gastric and bile simulations than the non-encapsulated or free probiotic.

“The survival rate of free probiotics was significantly reduced after exposure to simulated gastric and bile conditions, which confirms the loss of viability during transit to the action site in the diverse conditions of the tilapia intestine,”​ they said.

The findings should facilitate future use of the yeast probiotic, added the scientists.

Yeast experiment​

In the experiment, 90 fish were split into three groups and fed one of three diets. These included a control, the encapsulated yeast and free capsule yeast. Tests were run three times.

On days 0 and 30, fish from each group were sampled to record the amount of intestinal microorganisms including yeast, total bacteria and the lactic acid bacteria. Fish were weighed on day 0, day 14 and day 30 of the experiment to establish weight gain, growth rate and the feed conversion ratio.

On day 30, segments of intestine were collected from some fish getting each diet to measure intestinal fold length, record acidophilic granulocytes and determine mucous cells.

After the 30 days, fish were challenged with a strain of S. agalactiae​ and the mortality rate was recorded for 14 days. 

Results​

Fish fed the encapsulated diet had the largest weight gain and growth rate, said the researchers. That group also had the lowest feed conversion ratio.

Also, fish getting the encapsulated supplement had higher intestinal folds in the proximal and distal parts of the intestines compared to control, though no difference for the three groups was seen in the mid-intestine.

“The acidophilic granulocytes in the capsule group were higher than in the control and encapsulated S. cerevisiae groups in the proximal and middle parts of the intestines,”​ the team said. “The acidophilic granulocytes in the distal part of the intestine in the encapsulated S. cerevisiae group were higher than in the control and capsule groups. Intraepithelial lymphocytes in the encapsulated S. cerevisiae group were significantly greater than in the control and capsule groups in the proximal part of the intestine.”  ​

In the mucous cells, the encapsulated yeast had the lowest amount of cells in the mid-intestine and all other results were similar for each diet, the scientists said.

After the disease challenge, fish death ended on day three for fish getting the encapsulated yeast, while the control and capsule groups continued to die through days 11 and 14, they said. However, the mortality rates were not considered statistically significant, ranging from 70% mortality in the encapsulated group to 90% mortality in the control.

The yeast strain appears to not be effective against an oral streptococcal challenge, researchers concluded. But, the immune response generated might be important for bacterial pathogens.

“Further studies to examine the effects of encapsulated probiotic organisms on the immune response of fish following long-term storage should be conducted prior to practical application in aquaculture,”​ they added.


Sản phẩm khuyên dùng
Máy thổi khí AT-80 đang rất được ưa chuộng hiện nay trên thị trường, lưu lượng khí lớn, hoạt động mạnh mẽ.
Sản phẩm khuyên dùng
Tima - Tủ điều khiển ứng dụng mạng không dây LoRa. Tima sử dụng giao thức truyền dữ liệu không dây tầm xa LoRa điều khiển thiết bị như máy bơm nước, động cơ điện…
A cold cure for sick fish A cold cure for sick… Probiotics may offer Atlantic salmon, trout protection against cold-water disease Probiotics may offer Atlantic salmon,…